The birth of the operon.

Biofilm Formation and Detection of IcaAB Genes in Clinical Isolates of Methicillin Resistant Staphylococcus aureus

Objective(s) Methicillin-resistant Staphylococcus aureus (MRSA) is an important cause of nosocomial and community infections. Biofilm formation, mediated by a polysaccharide intercellular adhesin (PIA) and encoded by the ica operon, is considered to be an important virulence factor in both S. epidermidis and S. aureus. However, the clinical impact of the ica locus and PIA production is less w...

Cloning and expression of rhl AB operon under the control of tac promoter in E. coli

Today, efforts go towards the replacement of chemical surfactants by natural biological biosurfactants (biosurfactant), as these materials are not carcinogenic and highly compatibile with the environment. One of the main classes of biosurfactants is rhamnose containing glycolipid biosurfactant (rhamnolipids). This type of biosurfactants can be applied in many industries such as oil industry, ph...

Expression of acrA and acrB Genes in Esherichia coli Mutants with or without marR or acrR Mutations

  Objective(s): The major antibiotic efflux pump of Esherichia coli is AcrAB-TolC. The first part of the pump, AcrAB, is encoded by acrAB operon. The expression of this operon can be kept elevated by overexpression of an activator, MarA following inactivation of MarR and AcrR repressors due to mutation in encoding genes, marR and acrR, respectively. The aims of this research were to us...

Cloning and Sequencing of Desulfurization Operon froma Newly Isolated Bacterium Rhodococcus FMF

Molecular Study of Phase Variation of Type 1 Fimbriae in Uropathogenic Escherichia coli O44 Serotypes during Touching with Solid Surfaces

Background & Aims: Type 1 fimbriae is the most common adhesion factor in urine tract infection. In this Study, presence of virulence genes in isolated strains of uropathogenic E.Coli, O serotyping and molecular detection of phase variation of type 1 fimbriae were assessed during solid surfaces exposure. Methods: Isolated E.coli from urine samples of patients were serotyped by using serologic me...

PCR-MEDIATED CLONING A ND EXPRESSION OF THE GENE FOR THE B-SUBUNIT OF VIBRIO CHOLERAE TOXIN ISOLATED RECENTLY IN IRAN

Knowing the nucleotide sequence of the cholera toxin operon, we designed oligonucleotide primers for its-PCR amplification from local clinical isolates of V. cholerae. The resulting amplification product was cloned in a common pUC18 vector. Subsequently, a part of this operon encoding the cholera toxin Bsubunit (CTB) was reamplified and cloned between the BamH1 and EcoR1 sites of the same ...